Complete Biodegradation of 4-Fluorocinnamic Acid by a Consortium Comprising Arthrobacter sp. Strain G1 and Ralstonia sp. Strain H1

Syed A. Hasan, Maria Isabel M. Ferreira, Martijn J. Koetsier, Muhammad I. Arif, Dick B. Janssen*

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

19 Citaten (Scopus)
327 Downloads (Pure)


A consortium of the newly isolated bacterial strains Arthrobacter sp. strain G1 and Ralstonia sp. strain H1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. Strain G1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. In the presence of strain H1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. Degradation of 4-fluorocinnamic acid by strain G1 occurred through a beta-oxidation mechanism and started with the formation of 4-fluorocinnamoyl-coenzyme A (CoA), as indicated by the presence of 4-fluorocinnamoyl-CoA ligase. Enzymes for further transformation were detected in cell extract, i.e., 4-fluorocinnamoyl-CoA hydratase, 4-fluorophenyl-beta-hydroxy propionyl-CoA dehydrogenase, and 4-fluorophenyl-beta-keto propionyl-CoA thiolase. Degradation of 4-fluorobenzoic acid by strain H1 proceeded via 4-fluorocatechol, which was converted by an ortho-cleavage pathway.

Originele taal-2English
Pagina's (van-tot)572-579
Aantal pagina's8
TijdschriftApplied and environmental microbiology
Nummer van het tijdschrift2
StatusPublished - jan.-2011

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