TY - JOUR
T1 - Composition and architecture of biofilms on used voice prostheses
AU - Buijssen, Kevin J. D. A.
AU - van der Laan, Bernard F. A. M.
AU - van der Mei, Henny C.
AU - Atema-Smit, Jelly
AU - van den Huijssen, Pauline
AU - Busscher, Henk J.
AU - Harmsen, Hermie J. M.
PY - 2012/6
Y1 - 2012/6
N2 - Background Biofilms on medical devices are a frequent reason for failure of the device. Voice prostheses in laryngectomized patients deteriorate within 3 to 4 months due to adhering biofilms, impeding proper functioning. Recently, we showed that these biofilms are dominated by Candida and lactobacilli. However, the early report of this finding lacked an indepth analysis of the species diversity and community structure. Methods Denaturing gradient gel electrophoresis (DGGE) and sequence analysis of amplified rRNA genes was used to identify microorganisms. Fluorescence in situ hybridization (FISH) was used to analyze biofilm architecture. Results Candida are the predominant fungi in the biofilms, while lactobacilli are the predominant bacteria in all investigated biofilms, followed by streptococci. FISH shows that lactobacilli and streptococci seem to have an important interaction with fungi. Conclusion The results give a better understanding of biofilm formation and can be used in further development to prevent biofilm formation on voice prostheses. (c) 2011 Wiley Periodicals, Inc. Head Neck, 2011
AB - Background Biofilms on medical devices are a frequent reason for failure of the device. Voice prostheses in laryngectomized patients deteriorate within 3 to 4 months due to adhering biofilms, impeding proper functioning. Recently, we showed that these biofilms are dominated by Candida and lactobacilli. However, the early report of this finding lacked an indepth analysis of the species diversity and community structure. Methods Denaturing gradient gel electrophoresis (DGGE) and sequence analysis of amplified rRNA genes was used to identify microorganisms. Fluorescence in situ hybridization (FISH) was used to analyze biofilm architecture. Results Candida are the predominant fungi in the biofilms, while lactobacilli are the predominant bacteria in all investigated biofilms, followed by streptococci. FISH shows that lactobacilli and streptococci seem to have an important interaction with fungi. Conclusion The results give a better understanding of biofilm formation and can be used in further development to prevent biofilm formation on voice prostheses. (c) 2011 Wiley Periodicals, Inc. Head Neck, 2011
KW - biofilm
KW - voice prostheses
KW - lactobacillus
KW - Candida
KW - IN-SITU HYBRIDIZATION
KW - 16S RIBOSOMAL-RNA
KW - GEL-ELECTROPHORESIS ANALYSIS
KW - OLIGONUCLEOTIDE PROBES
KW - MICROBIAL-POPULATIONS
KW - RAPID IDENTIFICATION
KW - DIRECT VISUALIZATION
KW - SPATIAL-DISTRIBUTION
KW - SILICONE-RUBBER
KW - BACTERIA
U2 - 10.1002/hed.21833
DO - 10.1002/hed.21833
M3 - Article
SN - 1043-3074
VL - 34
SP - 863
EP - 871
JO - Head and Neck: Journal of the Sciences and Specialties of the Head and Neck
JF - Head and Neck: Journal of the Sciences and Specialties of the Head and Neck
IS - 6
ER -