Comprehensive analysis of miRNA expression in T-cell subsets of rheumatoid arthritis patients reveals defined signatures of naive and memory Tregs

Katarzyna Smigielska-Czepiel, A. van den Berg, P. Jellema, R. J. van der Lei, J. Bijzet, J. Kluiver, A. M. H. Boots, Liesbeth Brouwer, B-J Kroesen*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

95 Citaten (Scopus)
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Samenvatting

Disturbed expression of microRNAs (miRNAs) in regulatory T cells (Tregs) leads to development of autoimmunity in experimental mouse models. However, the miRNA expression signature characterizing Tregs of autoimmune diseases, such as rheumatoid arthritis (RA) has not been determined yet. In this study, we have used a microarray approach to comprehensively analyze miRNA expression signatures of both naive Tregs (CD4+CD45RO-CD25++) and memory Tregs (CD4+CD45RO+CD25++), as well as conventional naive (CD4+CD45RO+CD25+) and memory (CD4+CD45RO+CD25+) T cells (Tconvs) derived from peripheral blood of RA patients and matched healthy controls. Differential expression of selected miRNAs was validated by TaqMan-based quantitative reverse transcription-PCR. We found a positive correlation between increased expression of miR-451 in T cells of RA patients and disease activity score (DAS28), erythrocyte sedimentation rate levels and serum levels of interleukin-6. Moreover, we found characteristic, disease-and treatment-independent, global miRNA expression signatures defining naive Tregs, memory Tregs, naive Tconvs and memory Tconvs. The analysis allowed us to define miRNAs characteristic for a general naive phenotype (for example, miR-92a) and a general memory phenotype (for example, miR-21, miR-155). Importantly, the analysis allowed us to define miRNAs that are specifically expressed in both naive and memory Tregs, defining as such miRNA signature characterizing the Treg phenotype (that is, miR-146a, miR-3162, miR-1202, miR-1246 and miR-4281).

Originele taal-2English
Pagina's (van-tot)115-125
Aantal pagina's11
TijdschriftGENES AND IMMUNITY
Volume15
Nummer van het tijdschrift2
DOI's
StatusPublished - mrt-2014

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