Converting into an archaebacterium with a hybrid heterochiral membrane

Antonella Caforio, Melvin F Siliakus, Marten Exterkate, Samta Jain, Varsha R Jumde, Ruben L H Andringa, Servé W M Kengen, Adriaan J Minnaard, Arnold J M Driessen, John van der Oost

OnderzoeksoutputAcademicpeer review

32 Citaten (Scopus)
254 Downloads (Pure)

Samenvatting

One of the main differences between bacteria and archaea concerns their membrane composition. Whereas bacterial membranes are made up of glycerol-3-phosphate ester lipids, archaeal membranes are composed of glycerol-1-phosphate ether lipids. Here, we report the construction of a stable hybrid heterochiral membrane through lipid engineering of the bacteriumEscherichia coliBy boosting isoprenoid biosynthesis and heterologous expression of archaeal ether lipid biosynthesis genes, we obtained a viableE. colistrain of which the membranes contain archaeal lipids with the expected stereochemistry. It has been found that the archaeal lipid biosynthesis enzymes are relatively promiscuous with respect to their glycerol phosphate backbone and thatE. colihas the unexpected potential to generate glycerol-1-phosphate. The unprecedented level of 20-30% archaeal lipids in a bacterial cell has allowed for analyzing the effect on the mixed-membrane cell's phenotype. Interestingly, growth rates are unchanged, whereas the robustness of cells with a hybrid heterochiral membrane appeared slightly increased. The implications of these findings for evolutionary scenarios are discussed.

Originele taal-2English
Artikelnummerpnas.1721604115
Pagina's (van-tot)3704-3709
Aantal pagina's6
TijdschriftProceedings of the National Academy of Sciences of the United States of America
Volume115
Nummer van het tijdschrift14
Vroegere onlinedatum27-feb-2018
DOI's
StatusPublished - 3-apr-2018

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