Correlated Light Microscopy and Electron Microscopy

Klaas A. Sjollema*, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan, Ben N. G. Giepmans

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademic

33 Citaten (Scopus)

Samenvatting

Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Recent developments in fluorescence light microscopy (FLM) allow protein imaging in living cells and at the near molecular level.

However, fluorescence microscopy only reveals selected biomolecules or organelles but not the (ultra)structural context, as can be examined by electron microscopy (EM). LM and EM of the same cells, so-called correlative (or correlated) light and electron microscopy (CLEM), allow examining rare or dynamic events first by LM, and subsequently by EM. Here, we review progress in CLEM, with focus on matching the areas between different microscopic modalities. Moreover, we introduce a method that includes a virtual overlay and automated large-scale imaging, allowing to switch between most microscopes. Ongoing developments will revolutionize and standardize CLEM in the near future, which thus holds great promise to become a routine technique in cell biology.

Originele taal-2English
TitelCORRELATIVE LIGHT AND ELECTRON MICROSCOPY
RedacteurenT MullerReichert, P Verkade
Plaats van productieSAN DIEGO
UitgeverijAcademic Press
Pagina's157-173
Aantal pagina's17
ISBN van geprinte versie978-0-12-416026-2
DOI's
StatusPublished - 2012

Publicatie series

NaamMethods in Cell Biology
UitgeverijELSEVIER ACADEMIC PRESS INC
Volume111
ISSN van geprinte versie0091-679X

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