Counting stem cells: methodological constraints

Leonid V. Bystrykh, Evgenia Verovskaya, Erik Zwart, Mathilde Broekhuis, Gerald de Haan*

*Corresponding author voor dit werk

OnderzoeksoutputAcademicpeer review

67 Citaten (Scopus)

Samenvatting

The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

Originele taal-2English
Pagina's (van-tot)567-574
Aantal pagina's8
TijdschriftNature Methods
Volume9
Nummer van het tijdschrift6
DOI's
StatusPublished - jun.-2012

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