Coupled adhesion of bacteria to surfaces

Rebecca van der Westen Skogvold

Coupled adhesion of bacteria to surfaces

Vertaalde titel van de bijdrage: Gekoppelde hechting van bacteriën aan oppervlakken

Rebecca van der Westen Skogvold

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Adhesie van bacteriën vind plaats op alle natuurlijke en door de mens gemaakte oppervlakken en veroorzaakt een groot probleem binnen talrijke industriële en geneeskundige processen. Wanneer bacteriën een pathogene biofilm vormen op een oppervlak, zoals op biomaterialen, implantaten en apparaten, vormt dit een gevaar voor de gezondheid van de mens. Het controleren van hechting van bacteriën op deze oppervlakten is daarom belangrijk. Om meer inzicht te krijgen in de hechting van bacteriën op oppervlakken wordt de binding tussen bacteriën en oppervlakken bestudeerd. Voorheen dacht men dat deze binding niet flexibel was, maar recente studies hebben aangetoond dat deze binding beschouwd kan worden als viscoelastisch (flexibel). Het modelleren van deze viscoelastische binding tussen bacterie en oppervlak maakt het mogelijk om zowel de stijfheid als ook de viscoelasticiteit van de binding te bepalen. Hiermee wordt het duidelijk hoe deze bacterie-oppervlak verbinding kan worden beïnvloed en voorkomen. In dit proefschrift is met behulp van Quartz Crystal Microbalance en Total Internal Reflection Microscopy onderzocht hoe de viscoelasticiteit van de bacterie-oppervlak verbinding wordt bepaald door de ionsterkte van de vloeistof, de hydrofobiciteit (waterafstotendheid) van het oppervlak en de aanwezigheid of afwezigheid van oppervlakte structuren op het bacteriële celoppervlak.

Vertaalde titel van de bijdrage	Gekoppelde hechting van bacteriën aan oppervlakken
Originele taal-2	English
Kwalificatie	Doctor of Philosophy
Begeleider(s)/adviseur	Busscher, Henk, Supervisor van der Mei, Henny, Supervisor Sharma, Prashant, Co-supervisor van Loosdrecht, M, Beoordelingscommissie, Externe Persoon Ren, Y., Beoordelingscommissie, Externe Persoon Höök, F., Beoordelingscommissie, Externe Persoon
Datum van toekenning	25-jun.-2018
Plaats van publicatie	[Groningen]
Uitgever	Rijksuniversiteit Groningen
Gedrukte ISBN's	978-94-6299-980-0
Elektronische ISBN's	978-94-6375-014-1
Status	Published - 2018

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Title and contentsFinal publisher's version, 168 KB
Chapter 1Final publisher's version, 202 KB
Chapter 2Final publisher's version, 536 KB
Chapter 3Final publisher's version, 565 KB
Chapter 4Final publisher's version, 519 KB
Chapter 5Final publisher's version, 673 KB
Chapter 6Final publisher's version, 305 KB
Complete thesisFinal publisher's version, 2 MB
PropositionsFinal publisher's version, 46 KB

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title = "Coupled adhesion of bacteria to surfaces",

abstract = "Bacterial adhesion occurs on essentially all natural, as well as man-made surfaces and poses a major concern in engineering and medicine. Controlling initial bacterial adhesion is of key importance to prevent later problems after bacteria have grown into e.g. pathogenic biofilms on biomaterials, implants and devices that poses a threat to human health. Gaining insight into initial bacterial adhesion involves the study between bacteria and a substratum surface, more specifically the bond between the two latter. This bond has previously been thought to be rigid, although recent studies have shown that it is considered viscoelastic. Modeling this bacteria-surface bond as viscoelastic offers the opportunity to determine the stiffness as well as viscosity of the bond, thereby obtaining a more clear understanding as to how the bond can be influenced and moreover prevented. Through the means of Quartz Crystal Microbalance as well as Total Internal Reflection Microscopy this thesis explores how the viscoelasticity of the bond between bacteria-surface depends on environmental conditions, like ionic strength of the surrounding fluid, substratum hydrophobicity and absence or presence of binding tethers on a bacterial cell surface.",

author = "Skogvold, {Rebecca van der Westen}",

year = "2018",

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T1 - Coupled adhesion of bacteria to surfaces

AU - Skogvold, Rebecca van der Westen

PY - 2018

Y1 - 2018

N2 - Bacterial adhesion occurs on essentially all natural, as well as man-made surfaces and poses a major concern in engineering and medicine. Controlling initial bacterial adhesion is of key importance to prevent later problems after bacteria have grown into e.g. pathogenic biofilms on biomaterials, implants and devices that poses a threat to human health. Gaining insight into initial bacterial adhesion involves the study between bacteria and a substratum surface, more specifically the bond between the two latter. This bond has previously been thought to be rigid, although recent studies have shown that it is considered viscoelastic. Modeling this bacteria-surface bond as viscoelastic offers the opportunity to determine the stiffness as well as viscosity of the bond, thereby obtaining a more clear understanding as to how the bond can be influenced and moreover prevented. Through the means of Quartz Crystal Microbalance as well as Total Internal Reflection Microscopy this thesis explores how the viscoelasticity of the bond between bacteria-surface depends on environmental conditions, like ionic strength of the surrounding fluid, substratum hydrophobicity and absence or presence of binding tethers on a bacterial cell surface.

AB - Bacterial adhesion occurs on essentially all natural, as well as man-made surfaces and poses a major concern in engineering and medicine. Controlling initial bacterial adhesion is of key importance to prevent later problems after bacteria have grown into e.g. pathogenic biofilms on biomaterials, implants and devices that poses a threat to human health. Gaining insight into initial bacterial adhesion involves the study between bacteria and a substratum surface, more specifically the bond between the two latter. This bond has previously been thought to be rigid, although recent studies have shown that it is considered viscoelastic. Modeling this bacteria-surface bond as viscoelastic offers the opportunity to determine the stiffness as well as viscosity of the bond, thereby obtaining a more clear understanding as to how the bond can be influenced and moreover prevented. Through the means of Quartz Crystal Microbalance as well as Total Internal Reflection Microscopy this thesis explores how the viscoelasticity of the bond between bacteria-surface depends on environmental conditions, like ionic strength of the surrounding fluid, substratum hydrophobicity and absence or presence of binding tethers on a bacterial cell surface.

M3 - Thesis fully internal (DIV)

SN - 978-94-6299-980-0

PB - Rijksuniversiteit Groningen

CY - [Groningen]

ER -

Coupled adhesion of bacteria to surfaces

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