Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue GltTk

Albert Guskov, Sonja Jensen, Ignacio Faustino, Siewert J. Marrink, Dirk Jan Slotboom

Onderzoeksoutput: ArticleAcademicpeer review

76 Citaten (Scopus)
411 Downloads (Pure)

Samenvatting

Glutamate transporters catalyse the thermodynamically unfavourable transport of anionic amino acids across the cell membrane by coupling it to the downhill transport of cations. This coupling mechanism is still poorly understood, in part because the available crystal structures of these transporters are of relatively low resolution. Here we solve crystal structures of the archaeal transporter GltTk in the presence and absence of aspartate and use molecular dynamics simulations and binding assays to show how strict coupling between the binding of three sodium ions and aspartate takes place.
Originele taal-2English
Artikelnummer13420
Pagina's (van-tot)1-6
Aantal pagina's6
TijdschriftNature Communications
Volume7
DOI's
StatusPublished - 2016

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