TY - JOUR
T1 - CRISPR/Cas9
T2 - A powerful tool for identification of new targets for cancer treatment
AU - Liu, Bin
AU - Saber, Ali
AU - Haisma, Hidde J
N1 - Copyright © 2019. Published by Elsevier Ltd.
PY - 2019/4
Y1 - 2019/4
N2 - Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9), as a powerful genome-editing tool, has revolutionized genetic engineering. It is widely used to investigate the molecular basis of different cancer types. In this review, we present an overview of recent studies in which CRISPR/Cas9 has been used for the identification of potential molecular targets. Based on the collected data, we suggest here that CRISPR/Cas9 is an effective system to distinguish between mutant and wild-type alleles in cancer. We show that several new potential therapeutic targets, such as CD38, CXCR2, MASTL, and RBX2, as well as several noncoding (nc)RNAs have been identified using CRISPR/Cas9 technology. We also discuss the obstacles and challenges that we face for using CRISPR/Cas9 as a therapeutic.
AB - Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9), as a powerful genome-editing tool, has revolutionized genetic engineering. It is widely used to investigate the molecular basis of different cancer types. In this review, we present an overview of recent studies in which CRISPR/Cas9 has been used for the identification of potential molecular targets. Based on the collected data, we suggest here that CRISPR/Cas9 is an effective system to distinguish between mutant and wild-type alleles in cancer. We show that several new potential therapeutic targets, such as CD38, CXCR2, MASTL, and RBX2, as well as several noncoding (nc)RNAs have been identified using CRISPR/Cas9 technology. We also discuss the obstacles and challenges that we face for using CRISPR/Cas9 as a therapeutic.
U2 - 10.1016/j.drudis.2019.02.011
DO - 10.1016/j.drudis.2019.02.011
M3 - Review article
C2 - 30849442
VL - 24
SP - 955
EP - 970
JO - Drug Discovery Today
JF - Drug Discovery Today
SN - 1359-6446
IS - 4
ER -