Cryo-EM studies of membrane proteins at 200 keV

Chancievan Thangaratnarajah; Jan Rheinberger; Cristina Paulino

doi:10.1016/j.sbi.2022.102440

Cryo-EM studies of membrane proteins at 200 keV

Chancievan Thangaratnarajah^*, Jan Rheinberger, Cristina Paulino

^*Corresponding author voor dit werk

Onderzoeksoutput: Article › Academic › peer review

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Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful technique for the structural characterisation of membrane proteins, especially for targets previously thought to be intractable. Taking advantage of the latest hard- and software developments, high-resolution three-dimensional (3D) reconstructions of membrane proteins by cryo-EM has become routine, with 300-kV transmission electron microscopes (TEMs) being the current standard. The use of 200-kV cryo-TEMs is gaining increasingly prominence, showing the capabilities of reaching better than 2 Å resolution for soluble proteins and better than 3 Å resolution for membrane proteins. Here, we highlight the challenges working with membrane proteins and the impact of cryo-EM, and review the technical and practical benefits, achievements and limitations of imaging at lower electron acceleration voltages.

Originele taal-2	English
Artikelnummer	102440
Aantal pagina's	7
Tijdschrift	Current Opinion in Structural Biology
Volume	76
DOI's	https://doi.org/10.1016/j.sbi.2022.102440
Status	Published - 2022

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10.1016/j.sbi.2022.102440Licentie: CC BY

Cryo-EM studies of membrane proteins at 200 keVFinal publisher's version, 961 KBLicentie: CC BY

https://www.sciencedirect.com/science/article/pii/S0959440X22001191

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oLife Fellowship Programme
Roos, W. (Hoofdonderzoeker), van der Tak, F. (Hoofdonderzoeker), Zijlstra, W. (Hoofdonderzoeker), Dobos, V. (Postdoc), Heinen, L. (Postdoc), Thangaratnarajah, C. (Postdoc), Hoekzema, M. (Postdoc), Blokhuis, A. (Postdoc), Mascotti, L. (Postdoc), Padin Santos, D. (Postdoc), Chopra, A. (Postdoc), Obermaier, S. (Postdoc), Driver, M. (Postdoc), Moreira Goulart, M. (Postdoc), Sasidharan, S. (Postdoc), Samar Mahapatra, S. (Postdoc), Zylstra, A. (Postdoc), Geiger, Y. (Postdoc), Llopis Lorente, A. (Postdoc), Aschmann, D. (Postdoc) & Kulala Vittala, S. (Postdoc)
01/04/2019 → 31/03/2024
Project: Research

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title = "Cryo-EM studies of membrane proteins at 200 keV",

abstract = "Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful technique for the structural characterisation of membrane proteins, especially for targets previously thought to be intractable. Taking advantage of the latest hard- and software developments, high-resolution three-dimensional (3D) reconstructions of membrane proteins by cryo-EM has become routine, with 300-kV transmission electron microscopes (TEMs) being the current standard. The use of 200-kV cryo-TEMs is gaining increasingly prominence, showing the capabilities of reaching better than 2 {\AA} resolution for soluble proteins and better than 3 {\AA} resolution for membrane proteins. Here, we highlight the challenges working with membrane proteins and the impact of cryo-EM, and review the technical and practical benefits, achievements and limitations of imaging at lower electron acceleration voltages.",

author = "Chancievan Thangaratnarajah and Jan Rheinberger and Cristina Paulino",

year = "2022",

doi = "10.1016/j.sbi.2022.102440",

language = "English",

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journal = "Current Opinion in Structural Biology",

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AU - Thangaratnarajah, Chancievan

AU - Rheinberger, Jan

AU - Paulino, Cristina

PY - 2022

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N2 - Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful technique for the structural characterisation of membrane proteins, especially for targets previously thought to be intractable. Taking advantage of the latest hard- and software developments, high-resolution three-dimensional (3D) reconstructions of membrane proteins by cryo-EM has become routine, with 300-kV transmission electron microscopes (TEMs) being the current standard. The use of 200-kV cryo-TEMs is gaining increasingly prominence, showing the capabilities of reaching better than 2 Å resolution for soluble proteins and better than 3 Å resolution for membrane proteins. Here, we highlight the challenges working with membrane proteins and the impact of cryo-EM, and review the technical and practical benefits, achievements and limitations of imaging at lower electron acceleration voltages.

AB - Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful technique for the structural characterisation of membrane proteins, especially for targets previously thought to be intractable. Taking advantage of the latest hard- and software developments, high-resolution three-dimensional (3D) reconstructions of membrane proteins by cryo-EM has become routine, with 300-kV transmission electron microscopes (TEMs) being the current standard. The use of 200-kV cryo-TEMs is gaining increasingly prominence, showing the capabilities of reaching better than 2 Å resolution for soluble proteins and better than 3 Å resolution for membrane proteins. Here, we highlight the challenges working with membrane proteins and the impact of cryo-EM, and review the technical and practical benefits, achievements and limitations of imaging at lower electron acceleration voltages.

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DO - 10.1016/j.sbi.2022.102440

M3 - Article

SN - 0959-440X

VL - 76

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

M1 - 102440

ER -

Cryo-EM studies of membrane proteins at 200 keV

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