Cupriavidus necator as a model organism for CO2-based biotechnology

Halima Aliyu Alhafiz; Federico Di Bisceglie; Hannah Pia Franziska Meier; Isabell Weickardt; Itzel Andrea Castro González; Javier García Navarro; Pierre Schoenmakers; Sven Jerome Oyen; Elisabeth Lettau; Eric Lombard; Paul R.F. Cordero; Monika Konarzycka-Bessler; Nathalie Gorret; Sandy Schmidt; Stefan Schillberg; Marcel Mann; Stéphane E. Guillouet; Regina Kratzer; Lars Lauterbach

doi:10.1016/bs.mie.2025.01.017

Cupriavidus necator as a model organism for CO2-based biotechnology

Halima Aliyu Alhafiz^*, Federico Di Bisceglie, Hannah Pia Franziska Meier, Isabell Weickardt, Itzel Andrea Castro González, Javier García Navarro, Pierre Schoenmakers, Sven Jerome Oyen, Elisabeth Lettau, Eric Lombard, Paul R.F. Cordero, Monika Konarzycka-Bessler, Nathalie Gorret, Sandy Schmidt, Stefan Schillberg, Marcel Mann, Stéphane E. Guillouet, Regina Kratzer, Lars Lauterbach^*

^*Corresponding author voor dit werk

Chemische en Farmaceutische Biologie

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Cupriavidus necator H16 is a chemolithoautotrophic model organism that can grow in a simple mineral medium supplemented with the gases H2, O2 and CO2. This unique metabolic capability makes it a promising organism for sustainable biotechnology applications such as the production of bioplastics, single cell proteins and bulk chemicals from renewable resources. However, gas fermentation, particularly with explosive gas mixtures like H2 and O2, poses considerable technical and safety challenges. Effective control of gas mixtures, pressurized environments, mass transfer from gas to liquid and gas solubility is essential to ensure both reproducibility and safety in bioprocessing systems. This chapter describes a detailed methodology for the cultivation of C. necator across various micro, small- and medium-scale setups, while emphasizing safety protocols. The chapter also outlines analytical techniques for the quantification of polyhydroxybutyrate (PHB), single cell protein and isopropanol as product examples for gas fermentation.

Originele taal-2	English
Titel	Methods in Enzymology
Uitgeverij	Academic Press
Aantal pagina's	32
ISBN van geprinte versie	0076-6879
DOI's	https://doi.org/10.1016/bs.mie.2025.01.017
Status	E-pub ahead of print - 5-mrt.-2025

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Alhafiz, H. A., Di Bisceglie, F., Meier, H. P. F., Weickardt, I., González, I. A. C., Navarro, J. G., Schoenmakers, P., Oyen, S. J., Lettau, E., Lombard, E., Cordero, P. R. F., Konarzycka-Bessler, M., Gorret, N., Schmidt, S., Schillberg, S., Mann, M., Guillouet, S. E., Kratzer, R., & Lauterbach, L. (2025). Cupriavidus necator as a model organism for CO2-based biotechnology. In Methods in Enzymology Academic Press. Online publicatie vooraf. https://doi.org/10.1016/bs.mie.2025.01.017

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title = "Cupriavidus necator as a model organism for CO2-based biotechnology",

abstract = "Cupriavidus necator H16 is a chemolithoautotrophic model organism that can grow in a simple mineral medium supplemented with the gases H2, O2 and CO2. This unique metabolic capability makes it a promising organism for sustainable biotechnology applications such as the production of bioplastics, single cell proteins and bulk chemicals from renewable resources. However, gas fermentation, particularly with explosive gas mixtures like H2 and O2, poses considerable technical and safety challenges. Effective control of gas mixtures, pressurized environments, mass transfer from gas to liquid and gas solubility is essential to ensure both reproducibility and safety in bioprocessing systems. This chapter describes a detailed methodology for the cultivation of C. necator across various micro, small- and medium-scale setups, while emphasizing safety protocols. The chapter also outlines analytical techniques for the quantification of polyhydroxybutyrate (PHB), single cell protein and isopropanol as product examples for gas fermentation.",

keywords = "Gas fermentation, Biosynthesis, Polyhydroxybutyrate (PHB), Single cell protein, Molecular hydrogen, Carbon dioxide, Bulk chemicals",

author = "Alhafiz, {Halima Aliyu} and {Di Bisceglie}, Federico and Meier, {Hannah Pia Franziska} and Isabell Weickardt and Gonz{\'a}lez, {Itzel Andrea Castro} and Navarro, {Javier Garc{\'i}a} and Pierre Schoenmakers and Oyen, {Sven Jerome} and Elisabeth Lettau and Eric Lombard and Cordero, {Paul R.F.} and Monika Konarzycka-Bessler and Nathalie Gorret and Sandy Schmidt and Stefan Schillberg and Marcel Mann and Guillouet, {St{\'e}phane E.} and Regina Kratzer and Lars Lauterbach",

year = "2025",

month = mar,

day = "5",

doi = "10.1016/bs.mie.2025.01.017",

language = "English",

isbn = "0076-6879",

booktitle = "Methods in Enzymology",

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Alhafiz, HA, Di Bisceglie, F, Meier, HPF, Weickardt, I, González, IAC, Navarro, JG, Schoenmakers, P, Oyen, SJ, Lettau, E, Lombard, E, Cordero, PRF, Konarzycka-Bessler, M, Gorret, N, Schmidt, S, Schillberg, S, Mann, M, Guillouet, SE, Kratzer, R & Lauterbach, L 2025, Cupriavidus necator as a model organism for CO2-based biotechnology. in Methods in Enzymology. Academic Press. https://doi.org/10.1016/bs.mie.2025.01.017

TY - CHAP

T1 - Cupriavidus necator as a model organism for CO2-based biotechnology

AU - Alhafiz, Halima Aliyu

AU - Di Bisceglie, Federico

AU - Meier, Hannah Pia Franziska

AU - Weickardt, Isabell

AU - González, Itzel Andrea Castro

AU - Navarro, Javier García

AU - Schoenmakers, Pierre

AU - Oyen, Sven Jerome

AU - Lettau, Elisabeth

AU - Lombard, Eric

AU - Cordero, Paul R.F.

AU - Konarzycka-Bessler, Monika

AU - Gorret, Nathalie

AU - Schmidt, Sandy

AU - Schillberg, Stefan

AU - Mann, Marcel

AU - Guillouet, Stéphane E.

AU - Kratzer, Regina

AU - Lauterbach, Lars

PY - 2025/3/5

Y1 - 2025/3/5

N2 - Cupriavidus necator H16 is a chemolithoautotrophic model organism that can grow in a simple mineral medium supplemented with the gases H2, O2 and CO2. This unique metabolic capability makes it a promising organism for sustainable biotechnology applications such as the production of bioplastics, single cell proteins and bulk chemicals from renewable resources. However, gas fermentation, particularly with explosive gas mixtures like H2 and O2, poses considerable technical and safety challenges. Effective control of gas mixtures, pressurized environments, mass transfer from gas to liquid and gas solubility is essential to ensure both reproducibility and safety in bioprocessing systems. This chapter describes a detailed methodology for the cultivation of C. necator across various micro, small- and medium-scale setups, while emphasizing safety protocols. The chapter also outlines analytical techniques for the quantification of polyhydroxybutyrate (PHB), single cell protein and isopropanol as product examples for gas fermentation.

AB - Cupriavidus necator H16 is a chemolithoautotrophic model organism that can grow in a simple mineral medium supplemented with the gases H2, O2 and CO2. This unique metabolic capability makes it a promising organism for sustainable biotechnology applications such as the production of bioplastics, single cell proteins and bulk chemicals from renewable resources. However, gas fermentation, particularly with explosive gas mixtures like H2 and O2, poses considerable technical and safety challenges. Effective control of gas mixtures, pressurized environments, mass transfer from gas to liquid and gas solubility is essential to ensure both reproducibility and safety in bioprocessing systems. This chapter describes a detailed methodology for the cultivation of C. necator across various micro, small- and medium-scale setups, while emphasizing safety protocols. The chapter also outlines analytical techniques for the quantification of polyhydroxybutyrate (PHB), single cell protein and isopropanol as product examples for gas fermentation.

KW - Gas fermentation

KW - Biosynthesis

KW - Polyhydroxybutyrate (PHB)

KW - Single cell protein

KW - Molecular hydrogen

KW - Carbon dioxide

KW - Bulk chemicals

U2 - 10.1016/bs.mie.2025.01.017

DO - 10.1016/bs.mie.2025.01.017

M3 - Chapter

SN - 0076-6879

BT - Methods in Enzymology

PB - Academic Press

ER -

Cupriavidus necator as a model organism for CO2-based biotechnology

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