Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM

Stephan Rempel, Emanuela Colucci, Jan-Willem de Gier, Albert Guskov, Dirk Slotboom

OnderzoeksoutputAcademicpeer review

19 Citaten (Scopus)
307 Downloads (Pure)


Uptake of vitamin B12 is essential for many prokaryotes, but in most cases the membrane proteins involved are yet to be identified. We present the biochemical characterization and high-resolution crystal structure of BtuM, a predicted bacterial vitamin B12 uptake system. BtuM binds vitamin B12 in its base-off conformation, with a cysteine residue as axial ligand of the corrin cobalt ion. Spectroscopic analysis indicates that the unusual thiolate coordination allows for decyanation of vitamin B12. Chemical modification of the substrate is a property other characterized vitamin B12-transport proteins do not exhibit.

Originele taal-2English
Aantal pagina's8
TijdschriftNature Communications
Nummer van het tijdschrift1
StatusPublished - 2-aug-2018

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