Design and Directed Evolution of Enzymes for Iminium Biocatalysis

Guangcai Xu

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Samenvatting

The application of biocatalysis in conquering challenging synthesis requires the constant input of new enzymes. This thesis used directed evolution guided by catalytic promiscuity to develop a series of enzymes to perform iminium catalysis, a powerful catalysis mode originating from organocatalysis. Three such iminium biocatalysts have been created: a cofactor-independent peroxygenase achieving enantiocomplementary epoxidation reactions, and two efficient Michaelases, one based on a class I aldolase DERA and the other one based on an artificially tandem-fused tautomerase enzyme. These developments illustrate the power of combining chemomimetic enzyme design and directed evolution to create synthetically useful new-to-natural enzymes.
Originele taal-2English
KwalificatieDoctor of Philosophy
Toekennende instantie
  • Rijksuniversiteit Groningen
Begeleider(s)/adviseur
  • Poelarends, Gerrit, Supervisor
  • Quax, W. J., Supervisor
Datum van toekenning28-jun.-2022
Plaats van publicatie[Groningen]
Uitgever
DOI's
StatusPublished - 2022

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