Samenvatting
The application of biocatalysis in conquering challenging synthesis requires the constant input of new enzymes. This thesis used directed evolution guided by catalytic promiscuity to develop a series of enzymes to perform iminium catalysis, a powerful catalysis mode originating from organocatalysis. Three such iminium biocatalysts have been created: a cofactor-independent peroxygenase achieving enantiocomplementary epoxidation reactions, and two efficient Michaelases, one based on a class I aldolase DERA and the other one based on an artificially tandem-fused tautomerase enzyme. These developments illustrate the power of combining chemomimetic enzyme design and directed evolution to create synthetically useful new-to-natural enzymes.
Originele taal-2 | English |
---|---|
Kwalificatie | Doctor of Philosophy |
Toekennende instantie |
|
Begeleider(s)/adviseur |
|
Datum van toekenning | 28-jun.-2022 |
Plaats van publicatie | [Groningen] |
Uitgever | |
DOI's | |
Status | Published - 2022 |