Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution

Raimond B. G. Ravelli, Ruby D. Kalicharan, M. Cristina Avramut, Klaas A. Sjollema, Joachim W. Pronk, Freark Dijk, Abraham J. Koster, Jeroen T. J. Visser, Frank G. A. Faas, Ben N. G. Giepmans*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

30 Citaten (Scopus)
210 Downloads (Pure)

Samenvatting

Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.

Originele taal-2English
Artikelnummer1804
Aantal pagina's6
TijdschriftScientific Reports
Volume3
DOI's
StatusPublished - 8-mei-2013

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