Detection of G-quadruplex DNA in mammalian cells

Alexander Henderson, Yuliang Wu, Yu Chuan Huang, Elizabeth A. Chavez, Jesse Platt, F. Brad Johnson, Robert M. Brosh, Dipankar Sen, Peter M. Lansdorp*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

301 Citaten (Scopus)
352 Downloads (Pure)

Samenvatting

It has been proposed that guanine-rich DNA forms four-stranded structures in vivo called G-quadruplexes or G4 DNA. G4 DNA has been implicated in several biological processes, but tools to study G4 DNA structures in cells are limited. Here we report the development of novel murine monoclonal antibodies specific for different G4 DNA structures. We show that one of these antibodies designated 1H6 exhibits strong nuclear staining in most human and murine cells. Staining intensity increased on treatment of cells with agents that stabilize G4 DNA and, strikingly, cells deficient in FANCJ, a G4 DNA-specific helicase, showed stronger nuclear staining than controls. Our data strongly support the existence of G4 DNA structures in mammalian cells and indicate that the abundance of such structures is increased in the absence of FANCJ. We conclude that monoclonal antibody 1H6 is a valuable tool for further studies on the role of G4 DNA in cell and molecular biology.

Originele taal-2English
Pagina's (van-tot)860-869
Aantal pagina's10
TijdschriftNucleic Acids Research
Volume42
Nummer van het tijdschrift2
DOI's
StatusPublished - jan-2014

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