Objective: To determine a safe maximum number of artificial insemination (donor insemination [DI]) children per anonymous sperm donor.
Design: Multiparameter calculation model of contribution of DI to inbreeding.
Setting: Data of the collaborative DI centers and demographic population data of The Netherlands.
Interventions: Calculations of the contribution of DI to inbreeding were made, taking into account all possible unintentional consanguineous matings and consanguineous inseminations with a coefficient of inbreeding (F) of greater than or equal to 1/16 for the offspring.
Main Outcome Measure: Coefficient of inbreeding (F).
Results: The contribution of DI to inbreeding was estimated to be 46.6 X 10(-6) + k (1.66 X 10(-6)) (k = number of DI children per donor). When only half-sibling matings were considered, the k(max) was 32. When all possible consanguineous matings and inseminations were taken into account, k(max) decreased with 28% to 25 children.
Conclusion: Calculations of a maximum number of DI children per sperm donor, based on possible enhancement of inbreeding and given the Dutch population and DI setting, show that 25 children per DI donor is a safe threshold for clinical practice.