Development and application of CRISPR-based genetic tools in Bacillus species and Bacillus phages

Yafeng Song, Siqi He, Anita Jopkiewicz, Rita Setroikromo, Ronald van Merkerk, Wim J Quax

Onderzoeksoutputpeer review

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Samenvatting

Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool. Since its discovery as an adaptive immune system in prokaryotes, it has been applied in many different research fields including biotechnology and medical sciences. The high demand for rapid, highly efficient and versatile genetic tools to thrive in bacteria-based cell factories accelerates this process. This review mainly focuses on significant advancements of the CRISPR system in Bacillus subtilis, including the achievements in gene editing, and on problems still remaining. Next, we comprehensively summarize this genetic tool's up-to-date development and utilization in other Bacillus species, including B. licheniformis, B. methanolicus, B. anthracis, B. cereus, B. smithii and B. thuringiensis. Furthermore, we describe the current application of CRISPR tools in phages to increase Bacillus hosts' resistance to virulent phages and phage genetic modification. Finally, we suggest potential strategies to further improve this advanced technique and provide insights into future directions of CRISPR technologies for rendering Bacillus species cell factories more effective and more powerful.

Originele taal-2English
Pagina's (van-tot)2280-2298
Aantal pagina's19
TijdschriftJournal of Applied Microbiology
Volume133
Nummer van het tijdschrift4
Vroegere onlinedatum7-jul.-2022
DOI's
StatusPublished - okt.-2022

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