Dexamethasone coupled to albumin is selectively taken up by rat nonparenchymal liver cells and attenuates LPS-induced activation of hepatic cells

BN Melgert*, P Olinga, VK Jack, G Molema, Dirk Meijer, K Poelstra

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

48 Citaten (Scopus)

Samenvatting

Background/Aims: The human serum albumin (HSA) conjugate Dexa(10)-HSA was designed to specifically deliver the anti-inflammatory drug dexamethasone (Dexa) to nonparenchymal cells (NPC) in the rat liver. NPC play an important role in the pathogenesis of acute and chronic inflammatory liver diseases like fibrosis, Targeting Dexa to these cells might reduce its adverse effects and increase the efficacy.

Methods: Tissue and intrahepatic distributions of Dexa(10)-HSA were assessed in normal and fibrotic rats with I-125-labelled conjugate and by immunohistochemistry. The effect of the conjugate on lipopolysaccharide (LPS)-induced inflammation and cell activation was studied in vitro with precision-cut liver slices and in vivo.

Results: Ten minutes after i.v. injection 72+/-13% and 65+/-12% of a tracer dose of Dexa(10)-HSA had been taken up in normal and fibrotic livers, respectively. Unconjugated Dexa also accumulated in livers, but cellular distribution studies revealed an accumulation in parenchymal cells (NPC vs. parenchymal cell (PC) ratio=0.29+/-11, p

Conclusion: Dexa(10)-HSA was at least as effective as uncoupled Dexa at inhibiting LPS-induced effects, which indicates that HSA-bound Dexa is pharmacologically active. Coupling Dexa to HSA shifted the accumulation of Dexa from the PC to the NPC of livers. Since mediator release from NPC is crucial in the initiation and propagation of the fibrotic process, selective delivery of Dexa in NPC may improve the efficacy and safety of corticosteroid treatment of liver fibrosis.

Originele taal-2English
Pagina's (van-tot)603-611
Aantal pagina's9
TijdschriftJournal of Hepatology
Volume32
Nummer van het tijdschrift4
DOI's
StatusPublished - apr-2000

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