Direct immunofluorescence microscopy

Gilles F.H. Diercks*, Hendri H. Pas

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Direct immunofluorescence plays an important role in the diagnosis of autoimmune blistering diseases. The purpose of direct immunofluorescence microscopy is to detect in vivo antibodies in patient's skin or mucosa. Direct immunofluorescence of pemphigus shows depositions of immunoglobulins and/or complement on the epithelial cell surface of keratinocytes, whereas pemphigoid shows linear deposition of immunoglobulins along the epidermal basement membrane zone. This linear deposition can be separated in an n-serrated pattern and a u-serrated pattern. An n-serrated pattern is seen in blistering diseases with binding above the lamina densa with antibodies against hemidesmosomal components, e.g. bullous pemphigoid, while a u-serrated pattern points to a sublamina densa binding diseases caused by autoantibodies against type VII collagen, e.g. epidermolysis bullosa acquisita. Finally, dermatitis herpetiformis shows a granular IgA deposition along the epidermal basement membrane zone.

Originele taal-2English
TitelAutoimmune Bullous Diseases
SubtitelText and Review
UitgeverijSpringer International Publishing AG
Aantal pagina's8
ISBN van elektronische versie9783030915575
ISBN van geprinte versie9783030915568
StatusPublished - 19-jan.-2022

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