@article{c4a0c55cb6b849feb3826ed6fce0a84f,
title = "Directed Evolution of Enzymes: Library Screening Strategies",
abstract = "Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. (C) 2009 IUBMB",
keywords = "cell surface display, DNA shuffling, enzymology, error-prone PCR, high-throughput, in vitro compartmentalization, protein engineering, screening, saturation mutagenesis, CYCLODEXTRIN GLUCANOTRANSFERASE, CATALYTIC PROMISCUITY, BETA-GALACTOSIDASE, IN-VITRO, DNA, ENANTIOSELECTIVITY, BIOCATALYSIS, COMPARTMENTS, ELIMINATION, MUTATIONS",
author = "Hans Leemhuis and Kelly, {Ronan M.} and Lubbert Dijkhuizen",
year = "2009",
month = mar,
doi = "10.1002/iub.165",
language = "English",
volume = "61",
pages = "222--228",
journal = "Iubmb Life",
issn = "1521-6543",
number = "3",
}