Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells

Hilde M. van der Schaar*, Michael J. Rust, Chen Chen, Heidi van der Ende-Metselaar, Jan Wilschut, Xiaowei Zhuang, Jolanda M. Smit

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    379 Citaten (Scopus)
    274 Downloads (Pure)

    Samenvatting

    Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking, and fusion behavior of DENV. Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis. The virus particles move along the cell surface in a diffusive manner before being captured by a pre-existing clathrin-coated pit. Upon clathrin-mediated entry, DENV particles are transported to Rab5-positive endosomes, which subsequently mature into late endosomes through acquisition of Rab7 and loss of Rab5. Fusion of the viral membrane with the endosomal membrane was primarily detected in late endosomal compartments.

    Originele taal-2English
    Artikelnummer1000244
    Aantal pagina's9
    TijdschriftPLoS Pathogens
    Volume4
    Nummer van het tijdschrift12
    DOI's
    StatusPublished - 19-dec.-2008

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