Distinction of Staphylococcal Cassette Chromosome mec type V elements from Staphylococcus aureus ST398

Monika A. Chlebowicz, Thijs Bosch, Artur J. Sabat, Jan Paul Arends, Hajo Grundmann, Jan Maarten van Dijl*, Girbe Buist

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

3 Citaten (Scopus)

Samenvatting

Methicillin resistant S. aureus (MRSA) is a major threat for human health and well-being. In recent years, it has become clear that livestock is a potential reservoir for MRSA, most livestock-associated isolates belonging to the ST398 lineage. Importantly, ST398 strains were also reported as causative agents of severe invasive infections in humans with no evidence for livestock associations. Here we document the sequence of the J1 region of the type V (5C2&5) SCCmec element and its right chromosomal junction in the clinical PVL-positive ST398 MRSA isolate UMCG-M4. Sequence comparisons show that this SCCmec element and related type V elements from other S. aureus isolates share a common core structure, but differ substantially in the so-called J1 region. Additional PCR analyses and typing studies indicate that the J1 region of strain UMCG-M4 is specific for SCCmec elements of PVL-positive ST398 isolates. Lastly, we show that the sequenced right chromosomal junction is invariant in strains of the ST398 lineage. (C) 2013 Elsevier GmbH. All rights reserved.

Originele taal-2English
Pagina's (van-tot)422-432
Aantal pagina's11
TijdschriftInternational journal of medical microbiology
Volume303
Nummer van het tijdschrift8
DOI's
StatusPublished - dec-2013

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