Distinguishing aggregate formation and aggregate clearance using cell-based assays

Evelien Eenjes, Joanna M. Dragich, Harm H. Kampinga, Ai Yamamoto*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

20 Citaten (Scopus)
211 Downloads (Pure)

Samenvatting

The accumulation of ubiquitylated proteinaceous inclusions represents a complex process, reflecting the disequilibrium between aggregate formation and aggregate clearance. Although decreasing aggregate formation or augmenting aggregate clearance will ultimately lead to a diminished aggregate burden, in terms of disease pathogenesis, the different approaches can have distinct outcomes. Using a novel cell-based assay that can distinguish newly formed versus preformed inclusions, we demonstrate that two proteins previously implicated in the autophagic clearance of expanded polyglutamine inclusions, HspB7 and Alfy (also known as WDFY3), actually affect very distinct cellular processes to affect aggregate burden. Using this cell-based assay, we also establish that constitutive expression of the aggregation-prone protein can measurably slow the elimination of protein aggregates, given that not all aggregates appear to be available for degradation. This new assay can therefore not only determine at what step amodifiermight influence aggregate burden, but also can be used to provide new insights into how protein aggregates are targeted for degradation.
Originele taal-2English
Pagina's (van-tot)1260-1270
Aantal pagina's11
TijdschriftJournal of Cell Science
Volume129
Nummer van het tijdschrift6
DOI's
StatusPublished - 15-mrt-2016

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