Dynamic combinatorial chemistry to identify binders of ThiT, an S-component of the energy-coupling factor transporter for thiamine

Leticia Monjas, Lotteke J Y M Swier, Inda Setyawati, Dirk Jan Slotboom, Anna Katharina Herta Hirsch

OnderzoeksoutputAcademicpeer review

6 Citaten (Scopus)
257 Downloads (Pure)

Samenvatting

We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target.

Originele taal-2English
Pagina's (van-tot)1693-1696
Aantal pagina's4
TijdschriftChemMedChem
Volume12
Nummer van het tijdschrift20
DOI's
StatusPublished - 20-okt-2017

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