Engineered Proline-based Carboligases With Improved Catalytic Properties

Chao Guo

Onderzoeksoutput: Thesis fully internal (DIV)

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Using enzyme promiscuity to develop new biocatalysts is especially attractive for abiological reactions and can contribute to expanding our knowledge of the parameters involved in natural and laboratory enzyme evolution. A fascinating example of a catalytically promiscuous enzyme is 4-oxalocrotonate tautomerase (4-OT) from Pseudomonas putida mt-2, which utilizes an amino-terminal proline as key catalytic residue to promiscuously catalyze C-C bond-forming reactions, such as Michael additions and aldol condensations. The aim of the work described in this thesis was to further explore 4-OT for different synthetically useful C-C bond-forming reactions, as well as to improve its biocatalytic properties by mutability-landscape-guided protein engineering.
Originele taal-2English
KwalificatieDoctor of Philosophy
Toekennende instantie
  • Rijksuniversiteit Groningen
  • Poelarends, Gerrit, Supervisor
  • Quax, W. J., Supervisor
Datum van toekenning12-mrt-2021
Plaats van publicatie[Groningen]
StatusPublished - 2021

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