Enhanced protein production and methods thereof

Cristina Bongiorni (Uitvinder), Jolanda Neef (Uitvinder), Brian F. Schmidt (Uitvinder), Anita van Kimmenade (Uitvinder), Jan van Dijl (Uitvinder)

Onderzoeksoutput

Samenvatting

The present disclosure is generally related to modified Gram-positive bacterial cells producing increased amounts of one or more protein(s) of interest. Certain embodiments of the instant disclosure are therefore directed to modified Gram-positive bacterial cells expressing an increased amount of a POI relative to unmodified (i.e., parental) Gram-positive bacterial cells, wherein the modified (i.e., daughter) bacterial cells comprise a modification which increases rasP gene expression. In certain other embodiments, the disclosure pertains to methods of modifying bacterial cells such that the modified (daughter) cells produce an increased level of a protein of interest. In other embodiments, the disclosure pertains to a protein of interest produced by fermenting a modified bacterial cell of the instant disclosure. Certain other embodiments of the disclosure are directed to one or more proteinaceous compositions comprising one or more protein(s) of interest thus made.
Originele taal-2English
OctrooinummerWO2017112733
Prioriteitsdatum23/12/2015
StatusPublished - 29-jun.-2017

Citeer dit