Enhancement of the Immunogenicity and Protective Efficacy of a Mucosal Influenza Subunit Vaccine by the Saponin Adjuvant GPI-0100

Heng Liu, Harshad P. Patil, Jacqueline de Vries-Idema, Jan Wilschut, Anke Huckriede*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

22 Citaten (Scopus)
229 Downloads (Pure)

Samenvatting

Identification of safe and effective adjuvants remains an urgent need for the development of inactivated influenza vaccines for mucosal administration. Here, we used a murine challenge model to evaluate the adjuvant activity of GPI-0100, a saponin-derived adjuvant, on influenza subunit vaccine administered via the intranasal or the intrapulmonary route. Balb/c mice were immunized with 1 mu g A/PR/8 (H1N1) subunit antigen alone or in combination with varying doses of GPI-0100. The addition of GPI-0100 was required for induction of mucosal and systemic antibody responses to intranasally administered influenza vaccine and significantly enhanced the immunogenicity of vaccine administered via the intrapulmonary route. Remarkably, GPI-0100-adjuvanted influenza vaccine given at a low dose of 2x1 mu g either in the nares or directly into the lungs provided complete protection against homologous influenza virus infection.

Originele taal-2English
Artikelnummere52135
Aantal pagina's10
TijdschriftPLoS ONE
Volume7
Nummer van het tijdschrift12
DOI's
StatusPublished - 17-dec-2012

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