Enzymatic Network for Production of Ether Amines From Alcohols

Cyntia M. Palacio, Gica Ciprian Crismaru, Sebastian Bartsch, Vaidotas Navickas, Klaus Ditrich, Michael Breuer, Rohana Abu, John Woodley, Kai-Uwe Baldenius, Bian Wu, Dick Janssen

OnderzoeksoutputAcademicpeer review

18 Citaten (Scopus)

Samenvatting

We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed for reactions containing 10mM alcohol and up to 280mM ammonia corresponded well to predicted conversions. The results indicate that efficient amination can be driven by high concentrations of ammonia and may require improving enzyme robustness for scale-up. (C) 2016 Wiley Periodicals, Inc.

Originele taal-2English
Pagina's (van-tot)1853-1861
Aantal pagina's9
TijdschriftBiotechnology and Bioengineering
Volume113
Nummer van het tijdschrift9
DOI's
StatusPublished - sep-2016

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