ENZYME KINETICS FOR SYSTEMS BIOLOGY: WHEN, WHY AND HOW

Malgorzata Adamczyk*, Karen van Eunen, Barbara M. Bakker, Hans V. Westerhoff

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademic

12 Citaten (Scopus)

Samenvatting

In vitro enzymatic assays of cell-free extracts offer an opportunity to assess in vivo enzyme concentrations. If performed under conditions that resemble the conditions in vivo, they may also reveal some of the capacities and properties of the same enzymes in vivo; we shall call this the ex vivo approach. The kinetic characterization of purified enzymes has yet a different utility for systems biology, as does the in vivo determination of enzyme activities. All these approaches are different, and it is becoming important that the appropriate approach be used for the intended purpose. Here, we therefore discuss five approaches to the measurement of enzyme activity in terms of the source of the enzyme activity, the identity of the assay medium, and the purpose of the assay.

Originele taal-2English
TitelMETHODS IN ENZYMOLOGY, VOL 500
RedacteurenD Jameson, M Verma, HV Westerhoff
Plaats van productieSAN DIEGO
UitgeverijAcademic Press
Pagina's233-257
Aantal pagina's25
ISBN van geprinte versie978-0-12-385118-5
DOI's
StatusPublished - 2011

Publicatie series

NaamMethods in Enzymology
UitgeverijELSEVIER ACADEMIC PRESS INC
Volume500
ISSN van geprinte versie0076-6879

Citeer dit