Epigenome-wide association study identifies DNA methylation markers for asthma remission in whole blood and nasal epithelium

BIOS Consortium, Cancan Qi, Judith M Vonk, Diana A van der Plaat, Maartje A E Nieuwenhuis, F Nicole Dijk, Dylan Aïssi, Valérie Siroux, H Marike Boezen, Cheng-Jian Xu, Gerard H Koppelman*

*Corresponding author voor dit werk

OnderzoeksoutputAcademicpeer review

12 Citaten (Scopus)
85 Downloads (Pure)

Samenvatting

Background Asthma is a chronic respiratory disease which is not curable, yet some patients experience spontaneous remission. We hypothesized that epigenetic mechanisms may be involved in asthma remission. Methods Clinical remission (ClinR) was defined as the absence of asthma symptoms and medication for at least 12 months, and complete remission (ComR) was defined as ClinR with normal lung function and absence of airway hyperresponsiveness. We analyzed differential DNA methylation of ClinR and ComR comparing to persistent asthma (PersA) in whole blood samples (n = 72) and nasal brushing samples (n = 97) in a longitudinal cohort of well characterized asthma patients. Significant findings of whole blood DNA methylation were tested for replication in two independent cohorts, Lifelines and Epidemiological study on the Genetics and Environment of Asthma (EGEA). Results We identified differentially methylated CpG sites associated with ClinR (7 CpG sites) and ComR (129 CpG sites) in whole blood. One CpG (cg13378519, Chr1) associated with ClinR was replicated and annotated to PEX11 (Peroxisomal Biogenesis Factor 11 Beta). The whole blood DNA methylation levels of this CpG were also different between ClinR and healthy subjects. One ComR-associated CpG (cg24788483, Chr10) that annotated to TCF7L2 (Transcription Factor 7 Like 2) was replicated and associated with expression of TCF7L2 gene. One out of seven ClinR-associated CpG sites and 8 out of 129 ComR-associated CpG sites identified from whole blood samples showed nominal significance (P <0.05) and the same direction of effect in nasal brushes. Conclusion We identified DNA methylation markers possibly associated with clinical and complete asthma remission in nasal brushes and whole blood, and two CpG sites identified from whole blood can be replicated in independent cohorts and may play a role in peroxisome proliferation and Wnt signaling pathway.

Originele taal-2English
Artikelnummer60
Pagina's (van-tot)60
Aantal pagina's13
TijdschriftClinical and translational allergy
Volume10
Nummer van het tijdschrift1
DOI's
StatusPublished - 11-dec.-2020

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