Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

BIOS Consortium; Thanh T. Hoang; Cancan Qi; Kimberly C. Paul; Mikyeong Lee; Julie D. White; Marie Richards; Scott S. Auerbach; Stuart Long; Srishti Shrestha; Tianyuan Wang; Laura E. Beane Freeman; Jonathan N. Hofmann; Christine Parks; Cheng-Jian Xu; Beate Ritz; Gerard H. Koppelman; Stephanie J. London

doi:10.1289/EHP8928

Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

BIOS Consortium, Thanh T. Hoang, Cancan Qi, Kimberly C. Paul, Mikyeong Lee, Julie D. White, Marie Richards, Scott S. Auerbach, Stuart Long, Srishti Shrestha, Tianyuan Wang, Laura E. Beane Freeman, Jonathan N. Hofmann, Christine Parks, Cheng-Jian Xu, Beate Ritz, Gerard H. Koppelman, Stephanie J. London^*

^*Corresponding author voor dit werk

Groningen Research Institute for Asthma and COPD

Onderzoeksoutput › Academic › peer review

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BACKGROUND: Pesticide exposure is associated with many long-term health outcomes; the potential underlying mechanisms are not well established for most associations. Epigenetic modifications, such as DNA methylation, may contribute. Individual pesticides may be associated with specific DNA methylation patterns but no epigenome-wide association study (EWAS) has evaluated methylation in relation to individual pesticides.

OBJECTIVES: We conducted an EWAS of DNA methylation in relation to several pesticide active ingredients.

METHODS: The Agricultural Lung Health Study is a case-control study of asthma, nested within the Agricultural Health Study. We analyzed blood DNA methylation measured using Illumina's EPIC array in 1,170 male farmers of European ancestry. For pesticides still on the market at blood collection (2009-2013), we evaluated nine active ingredients for which at least 30 participants reported past and current (within the last 12 months) use, as well as seven banned organochlorines with at least 30 participants reporting past use. We used robust linear regression to compare methylation at individual C-phosphate-G sites (CpGs) among users of a specific pesticide to never users.

RESULTS: Using family-wise error rate (p < 9 x 10(-8)) or false-discovery rate (FDR

DISCUSSION: We identified differential methylation for several active ingredients in male farmers of European ancestry. These may serve as biomarkers of chronic exposure and could inform mechanisms of long-term health outcomes from pesticide exposure.

Originele taal-2	English
Artikelnummer	097008
Aantal pagina's	11
Tijdschrift	Environmental Health Perspectives
Volume	129
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.1289/EHP8928
Status	Published - sep.-2021

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BIOS Consortium, Hoang, T. T., Qi, C., Paul, K. C., Lee, M., White, J. D., Richards, M., Auerbach, S. S., Long, S., Shrestha, S., Wang, T., Freeman, L. E. B., Hofmann, J. N., Parks, C., Xu, C.-J., Ritz, B., Koppelman, G. H., & London, S. J. (2021). Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study. Environmental Health Perspectives, 129(9), Artikel 097008. https://doi.org/10.1289/EHP8928

@article{3a428c79992a4b519144dacf7cfede80,

title = "Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study",

abstract = "BACKGROUND: Pesticide exposure is associated with many long-term health outcomes; the potential underlying mechanisms are not well established for most associations. Epigenetic modifications, such as DNA methylation, may contribute. Individual pesticides may be associated with specific DNA methylation patterns but no epigenome-wide association study (EWAS) has evaluated methylation in relation to individual pesticides.OBJECTIVES: We conducted an EWAS of DNA methylation in relation to several pesticide active ingredients.METHODS: The Agricultural Lung Health Study is a case-control study of asthma, nested within the Agricultural Health Study. We analyzed blood DNA methylation measured using Illumina's EPIC array in 1,170 male farmers of European ancestry. For pesticides still on the market at blood collection (2009-2013), we evaluated nine active ingredients for which at least 30 participants reported past and current (within the last 12 months) use, as well as seven banned organochlorines with at least 30 participants reporting past use. We used robust linear regression to compare methylation at individual C-phosphate-G sites (CpGs) among users of a specific pesticide to never users.RESULTS: Using family-wise error rate (p < 9 x 10(-8)) or false-discovery rate (FDRDISCUSSION: We identified differential methylation for several active ingredients in male farmers of European ancestry. These may serve as biomarkers of chronic exposure and could inform mechanisms of long-term health outcomes from pesticide exposure.",

keywords = "EXPOSURE, ASSOCIATION, ATRAZINE, NEURONS",

author = "{BIOS Consortium} and Hoang, {Thanh T.} and Cancan Qi and Paul, {Kimberly C.} and Mikyeong Lee and White, {Julie D.} and Marie Richards and Auerbach, {Scott S.} and Stuart Long and Srishti Shrestha and Tianyuan Wang and Freeman, {Laura E. Beane} and Hofmann, {Jonathan N.} and Christine Parks and Cheng-Jian Xu and Beate Ritz and Koppelman, {Gerard H.} and London, {Stephanie J.}",

year = "2021",

month = sep,

doi = "10.1289/EHP8928",

language = "English",

volume = "129",

journal = "Environmental Health Perspectives",

issn = "0091-6765",

publisher = "US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE",

number = "9",

}

BIOS Consortium, Hoang, TT, Qi, C, Paul, KC, Lee, M, White, JD, Richards, M, Auerbach, SS, Long, S, Shrestha, S, Wang, T, Freeman, LEB, Hofmann, JN, Parks, C, Xu, C-J, Ritz, B, Koppelman, GH & London, SJ 2021, 'Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study', Environmental Health Perspectives, vol. 129, nr. 9, 097008. https://doi.org/10.1289/EHP8928

TY - JOUR

T1 - Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

AU - BIOS Consortium

AU - Hoang, Thanh T.

AU - Qi, Cancan

AU - Paul, Kimberly C.

AU - Lee, Mikyeong

AU - White, Julie D.

AU - Richards, Marie

AU - Auerbach, Scott S.

AU - Long, Stuart

AU - Shrestha, Srishti

AU - Wang, Tianyuan

AU - Freeman, Laura E. Beane

AU - Hofmann, Jonathan N.

AU - Parks, Christine

AU - Xu, Cheng-Jian

AU - Ritz, Beate

AU - Koppelman, Gerard H.

AU - London, Stephanie J.

PY - 2021/9

Y1 - 2021/9

N2 - BACKGROUND: Pesticide exposure is associated with many long-term health outcomes; the potential underlying mechanisms are not well established for most associations. Epigenetic modifications, such as DNA methylation, may contribute. Individual pesticides may be associated with specific DNA methylation patterns but no epigenome-wide association study (EWAS) has evaluated methylation in relation to individual pesticides.OBJECTIVES: We conducted an EWAS of DNA methylation in relation to several pesticide active ingredients.METHODS: The Agricultural Lung Health Study is a case-control study of asthma, nested within the Agricultural Health Study. We analyzed blood DNA methylation measured using Illumina's EPIC array in 1,170 male farmers of European ancestry. For pesticides still on the market at blood collection (2009-2013), we evaluated nine active ingredients for which at least 30 participants reported past and current (within the last 12 months) use, as well as seven banned organochlorines with at least 30 participants reporting past use. We used robust linear regression to compare methylation at individual C-phosphate-G sites (CpGs) among users of a specific pesticide to never users.RESULTS: Using family-wise error rate (p < 9 x 10(-8)) or false-discovery rate (FDRDISCUSSION: We identified differential methylation for several active ingredients in male farmers of European ancestry. These may serve as biomarkers of chronic exposure and could inform mechanisms of long-term health outcomes from pesticide exposure.

AB - BACKGROUND: Pesticide exposure is associated with many long-term health outcomes; the potential underlying mechanisms are not well established for most associations. Epigenetic modifications, such as DNA methylation, may contribute. Individual pesticides may be associated with specific DNA methylation patterns but no epigenome-wide association study (EWAS) has evaluated methylation in relation to individual pesticides.OBJECTIVES: We conducted an EWAS of DNA methylation in relation to several pesticide active ingredients.METHODS: The Agricultural Lung Health Study is a case-control study of asthma, nested within the Agricultural Health Study. We analyzed blood DNA methylation measured using Illumina's EPIC array in 1,170 male farmers of European ancestry. For pesticides still on the market at blood collection (2009-2013), we evaluated nine active ingredients for which at least 30 participants reported past and current (within the last 12 months) use, as well as seven banned organochlorines with at least 30 participants reporting past use. We used robust linear regression to compare methylation at individual C-phosphate-G sites (CpGs) among users of a specific pesticide to never users.RESULTS: Using family-wise error rate (p < 9 x 10(-8)) or false-discovery rate (FDRDISCUSSION: We identified differential methylation for several active ingredients in male farmers of European ancestry. These may serve as biomarkers of chronic exposure and could inform mechanisms of long-term health outcomes from pesticide exposure.

KW - EXPOSURE

KW - ASSOCIATION

KW - ATRAZINE

KW - NEURONS

U2 - 10.1289/EHP8928

DO - 10.1289/EHP8928

M3 - Article

SN - 0091-6765

VL - 129

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

IS - 9

M1 - 097008

ER -

Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

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