Erythropoietin stimulates normal endothelial progenitor cell-mediated endothelial turnover, but attributes to neovascularization only in the presence of local ischemia

B. Daan Westenbrink*, Hisko Oeseburg, Lennaert Kleijn, Pim van der Harst, Anne M. S. Belonje, Adriaan A. Voors, Regien G. Schoemaker, Rudolf A. De Boer, Dirk J. van Veldhuisen, Wiek H. van Gilst

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

53 Citaten (Scopus)

Samenvatting

Purpose We aimed to evaluate whether ischemia is required for erythropoietin (EPO) induced stimulation of endothelial progenitor cells (EPCs) and their related effects on endothelial and cardiac function.

Methods Bone marrow of rats was replaced by transgenic cells to allow tracking of EPCs. Ischemic heart failure was induced by left coronary artery ligation to induce myocardial infarction (MI) and control rats received a sham procedure. Three weeks after surgery, rats were randomized to receive EPO (darbepoetin alfa 40 mu g/kg per 3 weeks) or vehicle and were sacrificed 9 weeks after surgery.

Results In all treated groups, EPO significantly increased circulating EPCs and their incorporation into the endothelium of the ischemic and non-ischemic hearts as well as in the control organs; kidney and liver. This was associated with significantly improved endothelial function, which was strongly correlated with circulating EPCs (R=0.7, p

Conclusions In general, EPO stimulates normal endothelial progenitor cell-mediated endothelial turnover, but improves cardiac microvascularization and function only in the presence of ischemia.

Originele taal-2English
Pagina's (van-tot)265-274
Aantal pagina's10
TijdschriftCardiovascular Drugs and Therapy
Volume22
Nummer van het tijdschrift4
DOI's
StatusPublished - aug-2008

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