Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker; Anders R Larsen; Robert L Skov; Gavin K Paterson; Mark A Holmes; Artur J Sabat; Alexander W Friedrich; Robin Köck; Georg Peters; André Kriegeskorte

doi:10.1128/JCM.00075-13

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker^*, Anders R Larsen, Robert L Skov, Gavin K Paterson, Mark A Holmes, Artur J Sabat, Alexander W Friedrich, Robin Köck, Georg Peters, André Kriegeskorte

^*Bijbehorende auteur voor dit werk

Microbes in Health and Disease (MHD)

Onderzoeksoutput › Academic › peer review

20 Citaten (Scopus)

Samenvatting

A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

Originele taal-2	English
Pagina's (van-tot)	1917-1919
Aantal pagina's	3
Tijdschrift	Journal of Clinical Microbiology
Volume	51
Nummer van het tijdschrift	6
DOI's	https://doi.org/10.1128/JCM.00075-13
Status	Published - jun-2013

Toegang tot document

10.1128/JCM.00075-13

Citeer dit

Becker, Karsten ; Larsen, Anders R ; Skov, Robert L ; Paterson, Gavin K ; Holmes, Mark A ; Sabat, Artur J ; Friedrich, Alexander W ; Köck, Robin ; Peters, Georg ; Kriegeskorte, André. / Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, Nr. 6. blz. 1917-1919.

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abstract = "A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.",

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Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection. / Becker, Karsten; Larsen, Anders R; Skov, Robert L; Paterson, Gavin K; Holmes, Mark A; Sabat, Artur J ; Friedrich, Alexander W; Köck, Robin; Peters, Georg; Kriegeskorte, André.

In: Journal of Clinical Microbiology, Vol. 51, Nr. 6, 06.2013, blz. 1917-1919.

Onderzoeksoutput › Academic › peer review

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AU - Becker, Karsten

AU - Larsen, Anders R

AU - Skov, Robert L

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AU - Holmes, Mark A

AU - Sabat, Artur J

AU - Friedrich, Alexander W

AU - Köck, Robin

AU - Peters, Georg

AU - Kriegeskorte, André

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AB - A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

KW - CASSETTE CHROMOSOME MEC

KW - POLYMERASE-CHAIN-REACTION

KW - RAPID DETECTION

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KW - VARIANT

KW - IDENTIFICATION

KW - HOMOLOG

KW - STRAINS

KW - GERMANY

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