Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker; Anders R Larsen; Robert L Skov; Gavin K Paterson; Mark A Holmes; Artur J Sabat; Alexander W Friedrich; Robin Köck; Georg Peters; André Kriegeskorte

doi:10.1128/JCM.00075-13

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker^*, Anders R Larsen, Robert L Skov, Gavin K Paterson, Mark A Holmes, Artur J Sabat, Alexander W Friedrich, Robin Köck, Georg Peters, André Kriegeskorte

^*Corresponding author voor dit werk

Microbes in Health and Disease (MHD)

Onderzoeksoutput › Academic › peer review

23 Citaten (Scopus)

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A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

Originele taal-2	English
Pagina's (van-tot)	1917-1919
Aantal pagina's	3
Tijdschrift	Journal of Clinical Microbiology
Volume	51
Nummer van het tijdschrift	6
DOI's	https://doi.org/10.1128/JCM.00075-13
Status	Published - jun.-2013

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title = "Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection",

abstract = "A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.",

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author = "Karsten Becker and Larsen, {Anders R} and Skov, {Robert L} and Paterson, {Gavin K} and Holmes, {Mark A} and Sabat, {Artur J} and Friedrich, {Alexander W} and Robin K{\"o}ck and Georg Peters and Andr{\'e} Kriegeskorte",

year = "2013",

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doi = "10.1128/JCM.00075-13",

language = "English",

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AU - Becker, Karsten

AU - Larsen, Anders R

AU - Skov, Robert L

AU - Paterson, Gavin K

AU - Holmes, Mark A

AU - Sabat, Artur J

AU - Friedrich, Alexander W

AU - Köck, Robin

AU - Peters, Georg

AU - Kriegeskorte, André

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N2 - A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

AB - A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

KW - CASSETTE CHROMOSOME MEC

KW - POLYMERASE-CHAIN-REACTION

KW - RAPID DETECTION

KW - GENE

KW - VARIANT

KW - IDENTIFICATION

KW - HOMOLOG

KW - STRAINS

KW - GERMANY

KW - DENMARK

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DO - 10.1128/JCM.00075-13

M3 - Article

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SN - 0095-1137

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

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Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

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