Evaluation of [C-11]-DAA1106 for imaging and quantification of neuro inflammation in a rat model of herpes encephalitis

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Introduction: Many neurological and psychiatric disorders are associated with neuroinflammation. Positron emission tomography (PET) with [C-11]-PK11195 can be used to study neuroinflammation in these disorders. However; [C-11]-PK11195 may not be sensitive enough to visualize mild neuroinflammation. As a potentially more sensitive PET tracer for neuroinflammation, [C-11]-N-(2,5-dimethoxybenzyl)-N-(4-fluoro-2-phenoxyphenyl)-acetamide (DAA1106) was evaluated in a rat model of herpes encephalitis.

Methods: Male Wistar rats were intranasally inoculated with HSV-1 (HSE) or phosphate-buffered saline (control). At Day 6 or Day 7 after inoculation, small-animal [C-11]-DAA1106 PET scans were acquired, followed by ex vivo biodistribution. Arterial blood sampling was performed for quantification of uptake.

Results: In HSE rats, a significantly higher ex vivo, but not in vivo, uptake of [C-11]-DAA1106 was found in almost all examined brain areas (24-71%, P

Conclusions: [C-11]-DAA1106 showed a high and specific ex vivo uptake in the encephalitic rat brain. However, neuroinflammation could not be demonstrated in vivo by [C-11]-DAA1106 PET. Quantification of the uptake of [C-11]-DAA1106 using plasma sampling is not optimal, due to rapid tissue uptake, slow tissue clearance and low plasma activity. (C) 2010 Elsevier Inc. All rights reserved.

Originele taal-2English
Pagina's (van-tot)9-15
Aantal pagina's7
TijdschriftNuclear Medicine and Biology
Nummer van het tijdschrift1
StatusPublished - jan-2010

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