Evidence based selection of housekeeping genes

Hendrik J. M. de Jonge; Rudolf S. N. Fehrmann; Eveline S. J. M. de Bont; Robert M. W. Hofstra; Frans Gerbens; Willem A. Kamps; Elisabeth G. E. de Vries; Ate G. J. van der Zee; Gerard J. te Meerman; Arja ter Elst

doi:10.1371/journal.pone.0000898

Evidence based selection of housekeeping genes

Hendrik J. M. de Jonge, Rudolf S. N. Fehrmann, Eveline S. J. M. de Bont, Robert M. W. Hofstra, Frans Gerbens, Willem A. Kamps, Elisabeth G. E. de Vries, Ate G. J. van der Zee, Gerard J. te Meerman, Arja ter Elst^*

^*Corresponding author voor dit werk

Onderzoeksoutput › Academic › peer review

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For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e. g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e. g. RPS13, RPL27, RPS20 and OAZ1) with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes. In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species. Therefore, the identified novel candidate housekeeping genes seem to be the most appropriate choice for normalizing gene expression data.

Originele taal-2	English
Artikelnummer	898
Aantal pagina's	5
Tijdschrift	PLoS ONE
Volume	2
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.1371/journal.pone.0000898
Status	Published - 19-sep.-2007

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10.1371/journal.pone.0000898Licentie: CC BY

Evidence Based Selection of Housekeeping GenesFinal publisher's version, 212 KBLicentie: CC BY

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Data to: Evidence Based Selection of Housekeeping Genes
de Jonge, H. (Creator), Fehrmann, R. (Creator), de Bont, E. (Creator), Hofstra, R. M. W. (Creator), Gerbens, F. (Creator), Kamps, W. (Creator), de Vries, E. G. E. (Creator), van der Zee, A. (Creator), te Meerman, G. (Creator) & ter Elst, A. (Creator), University of Groningen, 19-sep.-2007
https://figshare.com/articles/Evidence_Based_Selection_of_Housekeeping_Genes/151673
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title = "Evidence based selection of housekeeping genes",

abstract = "For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e. g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e. g. RPS13, RPL27, RPS20 and OAZ1) with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes. In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species. Therefore, the identified novel candidate housekeeping genes seem to be the most appropriate choice for normalizing gene expression data.",

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author = "{de Jonge}, {Hendrik J. M.} and Fehrmann, {Rudolf S. N.} and {de Bont}, {Eveline S. J. M.} and Hofstra, {Robert M. W.} and Frans Gerbens and Kamps, {Willem A.} and {de Vries}, {Elisabeth G. E.} and {van der Zee}, {Ate G. J.} and {te Meerman}, {Gerard J.} and {ter Elst}, Arja",

year = "2007",

month = sep,

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doi = "10.1371/journal.pone.0000898",

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AU - de Jonge, Hendrik J. M.

AU - Fehrmann, Rudolf S. N.

AU - de Bont, Eveline S. J. M.

AU - Hofstra, Robert M. W.

AU - Gerbens, Frans

AU - Kamps, Willem A.

AU - de Vries, Elisabeth G. E.

AU - van der Zee, Ate G. J.

AU - te Meerman, Gerard J.

AU - ter Elst, Arja

PY - 2007/9/19

Y1 - 2007/9/19

N2 - For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e. g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e. g. RPS13, RPL27, RPS20 and OAZ1) with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes. In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species. Therefore, the identified novel candidate housekeeping genes seem to be the most appropriate choice for normalizing gene expression data.

AB - For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e. g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e. g. RPS13, RPL27, RPS20 and OAZ1) with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes. In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species. Therefore, the identified novel candidate housekeeping genes seem to be the most appropriate choice for normalizing gene expression data.

KW - TIME QUANTITATIVE PCR

KW - EXPRESSION

KW - RNA

KW - QUANTIFICATION

KW - MESSENGER

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JO - PLoS ONE

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Evidence based selection of housekeeping genes

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