To mimic (human) cholestasis in vitro requires multiple triggers to establish a diseased phenotype. However, this is currently not simulated by existing in vitro models. Therefore, there is a high need for multicellular systems similar to the human physiology. In such an in vitro model, cell-cell interactions and intact bile canaliculi with functional bile flow should be present and preserved during long-term culture. Precision-cut liver slices represent an ex vivo tissue culture technique that replicates most of the multicellular characteristics of a whole liver in vivo. This chapter describes the preparation and culturing of (human) precision-cut liver slices. Furthermore, a protocol to use the precision-cut liver slices technique to predict drug-induced cholestatic liver injury is described.

Originele taal-2English
TitelExperimental Cholestasis Research
RedacteurenMathieu Vinken
Plaats van productieNew York
UitgeverijHumana Press
Aantal pagina's12
ISBN van elektronische versie978-1-4939-9420-5
ISBN van geprinte versie978-1-4939-9419-9
StatusPublished - 2019

Publicatie series

NaamMethods in Molecular Biology
ISSN van geprinte versie1064-3745


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