Expanding the enzyme universe with genetically encoded unnatural amino acids

Ivana Drienovska*, Gerard Roelfes*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

32 Citaten (Scopus)
105 Downloads (Pure)


The emergence of robust methods to expand the genetic code allows incorporation of non-canonical amino acids into the polypeptide chain of proteins, thus making it possible to introduce unnatural chemical functionalities in enzymes. In this Perspective, we show how this powerful methodology is used to create enzymes with improved and novel, even new-to-nature, catalytic activities. We provide an overview of the current state of the art, and discuss the potential benefits of developing and using enzymes with genetically encoded non-canonical amino acids compared with enzymes containing only canonical amino acids.

Originele taal-2English
Pagina's (van-tot)193-202
Aantal pagina's10
TijdschriftNature Catalysis
Nummer van het tijdschrift3
StatusPublished - 6-jan-2020

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