Expression of a bioactive, single-chain choriogonadotropin in Dictyostelium discoideum

JC Heikoop, PDJ Grootenhuis*, M Blaauw, JS Veldema, PJM Van Haastert', MHK Linskens, Judith C. Heikoop, Jacob S. Veldema

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

30 Citaten (Scopus)
40 Downloads (Pure)

Samenvatting

Human choriogonadotropin (hCG) is a highly complex glycoprotein consisting of two non-covalently associated subunits. We aimed for the expression of a single-chain hCG in the soil amoebae Dictyostelium discoideum, a host which, in principle, provides simple genetics in combination with complex protein synthesis. To limit anticipated problems in mRNA translation, the first 30 bases of the coding sequence were altered to conform to the Dictyostelium preferred codon usage. We show that, immunologically, active single-chain hCG is indeed produced by Dictyostelium. Furthermore, this single-chain hCG is able to bind to the human luteinizing hormone/CG receptor and elicit a biological response. Its receptor-binding affinity is comparable to single-chain hCG produced by mammalian cells. We conclude that Dictyostelium is able to express bioactive highly complex heterologous glycoproteins.

Originele taal-2English
Pagina's (van-tot)359-363
Aantal pagina's5
TijdschriftEuropean Journal of Biochemistry
Volume256
Nummer van het tijdschrift2
DOI's
StatusPublished - 1-sep-1998

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