Fabrication of a Nitrogen and Boron-Doped Reduced Graphene Oxide Membrane-Less Amperometric Sensor for Measurement of Dissolved Oxygen in a Microbial Fermentation

Selvaraj Chinnathambi, Sumit Kumar, Gert-Jan Willem Euverink*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

1 Citaat (Scopus)
12 Downloads (Pure)


Dissolved oxygen (DO) is an important parameter to monitor in processes applicable in, for example, water technology and fermentation. In this study, we report the manufacturing of a membrane-less amperometric sensor that is based on the electrocatalytic reduction of oxygen. The sensor was tested in pH-neutral KNO3 solutions and in a microbial fermentation to monitor the consumption of dissolved oxygen. The nitrogen and boron-doped reduced graphene oxide (N,B-HRGO) is used as an electrocatalyst for oxygen reduction. One step co-doping of nitrogen and boron on graphene oxide is performed using the hydrothermal method. The sensor responded linearly to the DO concentration. A sensitivity of 0.2 mu A/mg.L-1 O-2 is obtained for the DO concentration of 1.5 to 10 mg.L-1 O-2. The membrane-less N,B-HRGO based DO sensor is successfully tested in an Amycolotopsis methanolica fermentation by monitoring the dissolved oxygen in real-time. The sensor detected the consumption of oxygen during the growth of A. methanolica, which shows the compatibility of N,B-HRGO as electrode material for amperometric measurement of dissolved oxygen in aerobic fermentation. This membrane-less amperometric sensor can be used to fabricate microdevices for microbioreactor applications.

Originele taal-2English
Aantal pagina's10
Nummer van het tijdschrift2
StatusPublished - jun-2020

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