Fecal Microbiota Composition Differs Between Children With beta-Cell Autoimmunity and Those Without

Marcus C. de Goffau, Kristiina Luopajarvi, Mikael Knip, Jorma Ilonen, Terhi Ruohtula, Taina Harkonen, Laura Orivuori, Saara Hakala, Gjalt W. Welling, Hermie J. Harmsen, Outi Vaarala*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

305 Citaten (Scopus)


The role of the intestinal microbiota as a regulator of autoimmune diabetes in animal models is well-established, but data on human type 1 diabetes are tentative and based on studies including only a few study subjects. To exclude secondary effects of diabetes and FILA risk genotype on gut microbiota, we compared the intestinal microbiota composition in children with at least two diabetes-associated autoantibodies (n = 18) with autoantibody-negative children matched for age, sex, early feeding history, and HLA risk genotype using pyrosequencing. Principal component analysis indicated that a low abundance of lactate-producing and butyrate-producing species was associated with beta-cell autoimmunity. In addition, a dearth of the two most dominant Bifidobacterium species, Bifidobacterium adolescentis and Bifidobacterium pseudocatenulatum, and an increased abundance of the Bacteroides genus were observed in the children with beta-cell autoimmunity. We did not find increased fecal calprotectin or IgA as marker of inflammation in children with beta-cell autoimmunity. Functional studies related to the observed alterations in the gut microbiome are warranted because the low abundance of bifidobacteria and butyrate-producing species could adversely affect the intestinal epithelial barrier function and inflammation, whereas the apparent importance of the Bacteroides genus in development of type 1 diabetes is insufficiently understood. Diabetes 62:1238-1244, 2013

Originele taal-2English
Pagina's (van-tot)1238-1244
Aantal pagina's7
Nummer van het tijdschrift4
StatusPublished - apr-2013

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