Fibrosis impairs the formation of new myofibers in the soft palate after injury

Paola L. Carvajal Monroy, Sander Grefte, Anne Marie Kuijpers-Jagtman, Maria P. A. C. Helmich, Frank A. D. T. G. Wagener, Johannes W. von den Hoff*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

7 Citaten (Scopus)


Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p

Originele taal-2English
Pagina's (van-tot)866-873
Aantal pagina's8
TijdschriftWound repair and regeneration
Nummer van het tijdschrift6
StatusPublished - 2015
Extern gepubliceerdJa

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