Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Lysates

Olga Sin, Alejandro Mata-Cabana, Renée I Seinstra, Ellen A A Nollen

    OnderzoeksoutputAcademicpeer review

    Samenvatting

    Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein's native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we describe in detail the filter retardation assay, a method that captures protein aggregates in a cellulose acetate membrane and allows its detection and quantification by immunoblotting.

    Originele taal-2English
    Artikelnummer3042
    Aantal pagina's9
    TijdschriftJMIR research protocols
    Volume8
    Nummer van het tijdschrift19
    DOI's
    StatusPublished - 5-okt-2018

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