FixNCut: single-cell genomics through reversible tissue fixation and dissociation

Laura Jiménez-Gracia, Domenica Marchese, Juan C Nieto, Ginevra Caratù, Elisa Melón-Ardanaz, Victoria Gudiño, Sara Roth, Kellie Wise, Natalie K Ryan, Kirk B Jensen, Xavier Hernando-Momblona, Joana P Bernardes, Florian Tran, Laura Katharina Sievers, Stefan Schreiber, Maarten van den Berge, Tessa Kole, Petra L van der Velde, Martijn C Nawijn, Philip RosenstielEduard Batlle, Lisa M Butler, Ian A Parish, Jasmine Plummer, Ivo Gut, Azucena Salas, Holger Heyn*, Luciano G Martelotto*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Samenvatting

The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.

Originele taal-2English
Artikelnummer81
Aantal pagina's36
TijdschriftGenome Biology
Volume25
DOI's
StatusPublished - 29-mrt.-2024

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