Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore

Yapei Tong, Misun Lee, Jeroen Drenth, Marco W. Fraaije*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

1 Citaat (Scopus)
74 Downloads (Pure)


Site-specific protein labeling methods are highly valuable tools for research and applications. We present a new protein labeling method that allows covalent attachment of a chromo-and fluorogenic flavin (FMN) to any targeted protein using a short flavinylation peptide-Tag. We show that this peptide can be as short as 7 residues and can be located at the N-Terminus, C-Terminus, or in internal regions of the target protein. Analogous to kinase-catalyzed phosphorylation, the flavin is covalently attached via a stable phosphothreonyl linkage. The site-specific covalent tethering of FMN is accomplished by using a bacterial flavin transferase. The covalent coupling of FMN was shown to work in Escherichia coli and Saccharomyces cerevisiae cells and could be performed in vitro, rendering the "Flavin-Tag"method a powerful tool for the selective decoration of proteins with a biocompatible redox-Active fluorescent chromophore.

Originele taal-2English
Pagina's (van-tot)1559-1563
Aantal pagina's5
Nummer van het tijdschrift8
StatusPublished - 18-aug.-2021

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