Samenvatting
The bacterial plasma membrane is an important cellular compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to a decrease in membrane fluidity that in turn leads to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. These alterations are reverted when membrane fluidity is restored by a chemical fluidizer. In vitro, the addition of a flotillin increases membrane fluidity of liposomes. Our data support a model in which flotillins are required for direct control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
Originele taal-2 | English |
---|---|
Artikelnummer | e57179 |
Pagina's (van-tot) | 1-21 |
Aantal pagina's | 21 |
Tijdschrift | eLife |
Volume | 9 |
DOI's | |
Status | Published - 14-jul-2020 |
Pers/Media
-
Special proteins regulate the fluidity of bacterial membranes
Aleksandra Zielinska & Dirk-Jan Scheffers
17/07/2020
1 item van Media-aandacht
Pers / media: Activiteiten met een maatschappelijk belang › Popular
-
Speciale eiwitten regelen de vloeibaarheid van bacteriemembraan
Aleksandra Zielinska & Dirk-Jan Scheffers
17/07/2020
1 item van Media-aandacht
Pers / media: Activiteiten met een maatschappelijk belang › Popular