TY - JOUR
T1 - Fluorescent Nanodiamonds for Tracking Single Polymer Particles in Cells and Tissues
AU - Li, Runrun
AU - Vedelaar, Thea A.
AU - Sigaeva, Alina
AU - Zhang, Yue
AU - Wu, Kaiqi
AU - Wang, Hui
AU - Wu, Xixi
AU - Olinga, Peter
AU - Wlodarzyk-Biegun, Małgorzata K.
AU - Schirhagl, Romana
N1 - Funding Information:
R.L. acknowledges financial support from the Chinese government via a CSC scholarship (No. 201906220229). R.S. is grateful for financial support from the NWO via a VIDI grant (016.Vidi.189.002) and MW via a VENI grant (VI.Veni.192.148).
Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
PY - 2023/9/5
Y1 - 2023/9/5
N2 - Polymer nanoparticles are widely used in drug delivery and are also a potential concern due to the increased burden of nano- or microplastics in the environment. In order to use polymer nanoparticles safely and understand their mechanism of action, it is useful to know where within cells and tissues they end up. To this end, we labeled polymer nanoparticles with nanodiamond particles. More specifically, we have embedded nanodiamond particles in the polymer particles and characterized the composites. Compared to conventional fluorescent dyes, these labels have the advantage that nanodiamonds do not bleach or blink, thus allowing long-term imaging and tracking of polymer particles. We have demonstrated this principle both in cells and entire liver tissues.
AB - Polymer nanoparticles are widely used in drug delivery and are also a potential concern due to the increased burden of nano- or microplastics in the environment. In order to use polymer nanoparticles safely and understand their mechanism of action, it is useful to know where within cells and tissues they end up. To this end, we labeled polymer nanoparticles with nanodiamond particles. More specifically, we have embedded nanodiamond particles in the polymer particles and characterized the composites. Compared to conventional fluorescent dyes, these labels have the advantage that nanodiamonds do not bleach or blink, thus allowing long-term imaging and tracking of polymer particles. We have demonstrated this principle both in cells and entire liver tissues.
UR - http://www.scopus.com/inward/record.url?scp=85169883755&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.3c01452
DO - 10.1021/acs.analchem.3c01452
M3 - Article
AN - SCOPUS:85169883755
SN - 0003-2700
VL - 95
SP - 13046
EP - 13054
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 35
ER -