Following nanoparticle uptake by cells using high-throughput microscopy and the deep-learning based cell identification algorithm Cellpose

Boxuan Yang, Ceri J Richards, Timea B. Gandek, Isa de Boer, Itxaso Aguirre Zuazo, Else Niemeijer, Christoffer Åberg

OnderzoeksoutputAcademicpeer review

1 Citaat (Scopus)
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Samenvatting

How many nanoparticles are taken up by human cells is a key question for many applications, both within medicine and safety. While many methods have been developed and applied to this question, microscopy-based methods present some unique advantages. However, the laborious nature of microscopy, in particular the consequent image analysis, remains a bottleneck. Automated image analysis has been pursued to remedy this situation, but offers its own challenges. Here we tested the recently developed deep-learning based cell identification algorithm Cellpose on fluorescence microscopy images of HeLa cells. We found that the algorithm performed very well, and hence developed a workflow that allowed us to acquire, and analyse, thousands of cells in a relatively modest amount of time, without sacrificing cell identification accuracy. We subsequently tested the workflow on images of cells exposed to fluorescently-labelled polystyrene nanoparticles. This dataset was then used to study the relationship between cell size and nanoparticle uptake, a subject where high-throughput microscopy is of particular utility.
Originele taal-2English
Aantal pagina's13
TijdschriftFrontiers in Nanotechnology
Volume5
DOI's
StatusPublished - 18-mei-2023

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