Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillus subtilis

Margarita Bernal-Cabas, Marcus Miethke, Minia Antelo-Varela, Rocío Aguilar Suárez, Jolanda Neef, Larissa Schön, Giorgio Gabarrini, Andreas Otto, Dörte Becher, Diana Wolf, Jan Maarten van Dijl*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

4 Citaten (Scopus)
82 Downloads (Pure)


The bacterial twin-arginine (Tat) pathway serves in the exclusive secretion of folded proteins with bound co-factors. While Tat pathways in Gram-negative bacteria and chloroplast thylakoids consist of conserved TatA, TatB and TatC subunits, the Tat pathways of Bacillus species and many other Gram-positive bacteria stand out for their minimalist nature with the core translocase being composed of essential TatA and TatC subunits only. Here we addressed the question whether the minimal TatAyCy translocase of Bacillus subtilis recruits additional cellular components that modulate its activity. To this end, TatAyCy was purified by affinity- and size exclusion chromatography, and interacting co-purified proteins were identified by mass spectrometry. This uncovered the cell envelope stress responsive LiaH protein as an accessory subunit of the TatAyCy complex. Importantly, our functional studies show that Tat expression is tightly trailed by LiaH induction, and that LiaH itself determines the capacity and quality of TatAyCy-dependent protein translocation. In contrast, LiaH has no role in high-level protein secretion via the general secretion (Sec) pathway. Altogether, our observations show that protein translocation by the minimal Tat translocase TatAyCy is tightly intertwined with an adequate bacterial response to cell envelope stress. This is consistent with a critical need to maintain cellular homeostasis, especially when the membrane is widely opened to permit passage of large fully-folded proteins via Tat.

Originele taal-2English
Aantal pagina's15
TijdschriftBiochimica et Biophysica Acta - Molecular Cell Research
Nummer van het tijdschrift8
Vroegere onlinedatum14-apr-2020
StatusPublished - aug-2020

Citeer dit