Genetic instability from a single S phase after whole-genome duplication

Simon Gemble*, René Wardenaar, Kristina Keuper, Nishit Srivastava, Maddalena Nano, Anne-Sophie Macé, Andréa E Tijhuis, Sara Vanessa Bernhard, Diana C J Spierings, Anthony Simon, Oumou Goundiam, Helfrid Hochegger, Matthieu Piel, Floris Foijer, Zuzana Storchová, Renata Basto*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

39 Citaten (Scopus)
86 Downloads (Pure)


Diploid and stable karyotypes are associated with health and fitness in animals. By contrast, whole-genome duplications-doublings of the entire complement of chromosomes-are linked to genetic instability and frequently found in human cancers(1-3). It has been established that whole-genome duplications fuel chromosome instability through abnormal mitosis(4-8); however, the immediate consequences of tetraploidy in the first interphase are not known. This is a key question because single whole-genome duplication events such as cytokinesis failure can promote tumorigenesis(9). Here we find that human cells undergo high rates of DNA damage during DNA replication in the first S phase following induction of tetraploidy. Using DNA combing and single-cell sequencing, we show that DNA replication dynamics is perturbed, generating under- and over-replicated regions. Mechanistically, we find that these defects result from a shortage of proteins during the G1/S transition, which impairs the fidelity of DNA replication. This work shows that within a single interphase, unscheduled tetraploid cells can acquire highly abnormal karyotypes. These findings provide an explanation for the genetic instability landscape that favours tumorigenesis after tetraploidization.

Originele taal-2English
Pagina's (van-tot)146–151
Aantal pagina's29
Vroegere onlinedatum30-mrt.-2022
StatusPublished - apr.-2022

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