Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq

Clemence Claussin, David Porubsky, Diana C. J. Spierings, Nancy Halsema, Stefan Rentas, Victor Guryev, Peter M. Lansdorp, Michael Chang

OnderzoeksoutputAcademicpeer review

21 Citaten (Scopus)
288 Downloads (Pure)


Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.

Originele taal-2English
Aantal pagina's17
StatusPublished - 12-dec.-2017

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