Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq

Clemence Claussin, David Porubsky, Diana C. J. Spierings, Nancy Halsema, Stefan Rentas, Victor Guryev, Peter M. Lansdorp, Michael Chang

OnderzoeksoutputAcademicpeer review

11 Citaten (Scopus)
226 Downloads (Pure)

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Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.

Originele taal-2English
Artikelnummer30560
Aantal pagina's17
TijdschrifteLife
Volume6
DOI's
StatusPublished - 12-dec-2017

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